Catalog Number - 59300-04

MicroBee Amino™ is a traditional aminopropyl HPLC stationary phase developed for laboratories requiring a proven alternative to legacy amino column methods. As part of the Cogent™ Similars product line, it has been widely used for many years in carbohydrate analysis, normal phase chromatography, and other applications where amino selectivity is desired. The stationary phase consists of a bonded amino functional group attached to high-purity Type-A silica, providing retention characteristics comparable to traditional amino columns used in established methods.

59300-04 is a 300 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Amino particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ Amino and similar columns, making it particularly useful for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for challenging separations, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L8 column is an excellent alternative for methods specifying Microbondapak™ Amino and similar stationary phases. Packed and shipped in normal phase solvents, it is optimized for normal phase chromatography and related separations. The aminopropyl functionality provides selectivity useful for carbohydrate analysis, sugar profiling, geometric isomer separations, and other applications where traditional amino phases continue to be preferred. As one of the most popular MicroBee Amino column configurations, it is frequently selected when maximum resolution is required from a legacy amino method.

Key Benefits

• Traditional aminopropyl stationary phase

• Suitable alternative for USP L8 methods

• Compatible with many legacy Microbondapak™ methods

• Maximum resolution from 300 mm column length

• Optimized for normal phase chromatography

• Suitable for carbohydrate and sugar analysis

• 125Å pore size for broad analyte compatibility

• One of the most popular MicroBee Amino columns

Typical Applications

• USP L8 methods

• Normal phase HPLC

• Carbohydrate analysis

• Sugar separations

• Food and beverage testing

• Pharmaceutical analysis

• Method transfer and validation

• Geometric isomer separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Amino

• USP Classification: L8

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Aminopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Shipping Solvent: Normal Phase

• Brand: Cogent

Important Notes

• One of the most popular MicroBee Amino column configurations

• Packed and shipped in normal phase solvents

• Designed primarily for normal phase chromatography

• USP L8 column classification

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Extended column length provides maximum resolving power

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